Journal: Retrovirology
Article Title: Intestinal endothelial cells increase HIV infection and latency in resting and activated CD4 + T cells, particularly affecting CCR6 + CD4 + T cells
doi: 10.1186/s12977-023-00621-y
Figure Lengend Snippet: Fig. 1 IEC stimulation increases HIV infection of resting CD4 + T cells. Here and throughout the paper, boxplots show the median (horizontal line), and the central box extends to the 25th and 75th percentiles. The whiskers then extend 1.5 inter-quartile ranges or to the minimum or maximum observed value, whichever is closer to the median. A Infection rates in T cells stimulated with endothelial cells. Resting T cells were cultured alone (Resting), or co-cultured with human umbilical vein endothelial cells (EC), human lymphatic endothelial cells (LEC), or human intestinal endothelial cells (IEC). + and − indicate treatment with or without IFN-γ respectively in EC, LEC, or IEC. All T cells were infected with an HIV reporter virus expressing GFP 1 day after co-culture, and the %GFP+ cells were measured on day 6 post-infection. Samples were taken in triplicate for each donor, and different donors are represented by different symbols (n = 5). P-values are from post-hoc pairwise comparisons of marginal means based on beta generalized linear models (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). B Expression of activation markers versus GFP expression in IEC + stimulated resting CD4 + T cells. Similar to A, but on day 6 post-infection, T cells were stained for three activation markers: CD25, CD69, and HLA-DR. C Expression of activation markers and infection rates in T cells with and without IEC stimulation (n = 3), similar to ( B ). D Infection rates in resting memory and naïve T cells stimulated with IEC. Memory and naïve T cells were isolated, and the rest of the experimental procedures are the same as ( A ). Samples were taken in triplicate for each donor, and different donors are represented by different symbols (n = 3). P-values are from post-hoc pairwise comparisons of marginal means based on beta generalized linear models (****p<0.0001).
Article Snippet: LEC were obtained from ScienCell Research Laboratories (isolated from human lymph nodes) and cultured in basal endothelial cell medium supplemented with 5% fetal bovine serum (FBS) and 1% penicillin/streptomycin solution (P/S) (Invitrogen).
Techniques: Infection, Cell Culture, Virus, Expressing, Co-Culture Assay, Activation Assay, Staining, Isolation
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